Application
HeLa whole cell and nuclear extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with PHF10 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using PHF10 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.Application
Immunohistochemical analysis of paraffin-embedded Hela xenograft, using PHF10 antibody at 1:500 dilution.Application
PHF10 antibody detects PHF10 protein by western blot analysis. A.50 µg mouse colon lysate/extract 10% SDS-PAGE PHF10 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
PHF10 antibody detects PHF10 protein at nucleus in mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. PHF10 antibody diluted at 1:500.Application
PHF10 antibody detects PHF10 protein at nucleus in rat lung by immunohistochemical analysis. Sample: Paraffin-embedded rat lung. PHF10 antibody diluted at 1:500.| Product Name | PHF10 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PHF10 (PHD finger protein 10)This gene contains a predicted ORF that encodes a protein with two zinc finger domains. The function of the encoded protein is not known. Sequence analysis suggests that multiple alternatively spliced transcript variants are derived from this gene but the full-length nature of only two of them is known. These two splice variants encode different isoforms. A pseudogene for this gene is located on Xq28. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PHF10. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, BovineRat (94%), Bovine (80%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | BAF45A antibody FLJ10975 antibody MGC111009 antibody XAP135 antibody PHF10 antibody BRG1-associated factor 45a antibody PHD finger protein 10 antibody PHD finger protein 10 isoform a antibody PHD zinc finger protein XAP135 antibody BRG1-associate |
|---|---|
| Molecular Weight(MW) | 56 kDa (note) (3) |
| Cellular Localization | Nucleus |
Application
HeLa whole cell and nuclear extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with PHF10 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using PHF10 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.
Application
Immunohistochemical analysis of paraffin-embedded Hela xenograft, using PHF10 antibody at 1:500 dilution.
Application
PHF10 antibody detects PHF10 protein by western blot analysis. A.50 µg mouse colon lysate/extract 10% SDS-PAGE PHF10 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
PHF10 antibody detects PHF10 protein at nucleus in mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. PHF10 antibody diluted at 1:500.
Application
PHF10 antibody detects PHF10 protein at nucleus in rat lung by immunohistochemical analysis. Sample: Paraffin-embedded rat lung. PHF10 antibody diluted at 1:500.| Positive Control | HeLa , mouse colon |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
