Application
PHF2 antibody detects PHF2 protein by western blot analysis. A. 30 μg 293T whole cell extract B. 30 μg A431 whole cell extract C. 30 μg HeLa whole cell extract D. 30 μg HepG2 whole cell extract 5 % SDS-PAGE PHF2 antibody dilution: 1:1000| Product Name | PHF2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PHF2 (PHD finger protein 2)Probable regulator of histone methyltransferase complexes. Recruited to trimethylated 'Lys-4' of histone H3 (H3K4me3) at rDNA promoters and promotes expression of rDNA. Was reported to have histone demethylase activity by itself and specifically demethylate monomethylated 'Lys-9' of histone H3 (H3K9me1), an epigenetic repressive mark. However, this activity is unclear in vivo and requires additional evidence. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PHF2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | GRC5 CENP-35 JHDM1E PHD finger protein 2 |
|---|---|
| Target | PHF2 |
| Molecular Weight(MW) | 121 kDa (note) |
| Cellular Localization | Nucleus |
Application
PHF2 antibody detects PHF2 protein by western blot analysis. A. 30 μg 293T whole cell extract B. 30 μg A431 whole cell extract C. 30 μg HeLa whole cell extract D. 30 μg HepG2 whole cell extract 5 % SDS-PAGE PHF2 antibody dilution: 1:1000| Positive Control | 293T, A431, HeLa, HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
