Application
Sample (30 ug of whole cell lysate) A: NT2D1 B: SK-N-SH 10% SDS PAGE diluted at 1:1000| Product Name | PHF7 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PHF7 (PHD finger protein 7)Spermatogenesis is a complex process regulated by extracellular and intracellular factors as well as cellular interactions among interstitial cells of the testis, Sertoli cells, and germ cells. In the testis, this gene is expressed in Sertoli cells but not germ cells. However, this gene is not expressed in a patient who exhibited spermatogenic arrest at the spermatocyte stage. Spermatogenic arrest is an interruption of germ cell differentiation that may result in oligospermia or azoospermia. The proteins encoded by this gene contain plant homeodomain (PHD) finger domains, also known as leukemia associated protein (LAP) domains, believed to be involved in transcriptional regulation. Thus this protein, which localizes to the nucleus of transfected cells, has been implicated in the transcriptional regulation of spermatogenesis. Two protein isoforms are encoded by transcript variants of this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PHF7. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Rat, Rhesus Monkey, ChimpanzeeRat (83%), Rhesus Monkey (99%), Chimpanzee (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp434L1850 antibody HSPC045 antibody HSPC226 antibody MGC26088 antibody NYD-SP6 antibody PHF7 antibody PHD finger protein 7 antibody testis development protein NYD-SP6 antibody |
|---|---|
| Molecular Weight(MW) | 44 kDa (note) (4) |
| Cellular Localization | Nucleus |
Application
Sample (30 ug of whole cell lysate) A: NT2D1 B: SK-N-SH 10% SDS PAGE diluted at 1:1000| Positive Control | NT2D1 , SK-N-SH |
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| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
