Application
PHKA1 antibody detects PHKA1 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with PHKA1 antibody diluted by 1:500.Application
PHKA1 antibody detects PHKA1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: PHKA1 protein stained by PHKA1 antibody diluted at 1:200. Blue: Hoechst 33342 staining.| Product Name | PHKA1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PHKA1 (phosphorylase kinase, alpha 1 (muscle))The PHKA1 gene encodes the alpha subunit of muscle phosphorylase kinase (EC 2.7.1.38), a key regulatory enzyme of glycogen metabolism. Phosphorylase kinase consists of 4 copies of an alpha-beta-gamma-delta tetramer. The alpha, beta (PHKB; MIM 172490), and gamma (PHKG1; MIM 172470 and PHKG2; MIM 172471) subunits have several isoforms; the delta subunit is calmodulin (CALM1; MIM 114180). PHKA2 (MIM 306000) encodes the alpha subunit of liver-specific phosphorylase kinase and is also located on the X chromosome.[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PHKA1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Rhesus MonkeyMouse (86%), Rat (86%), Rhesus Monkey (96%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | phosphorylase kinase alpha 1 (muscle) PHKA |
|---|---|
| Target | PHKA1 |
| Molecular Weight(MW) | 137 kDa (note) (2) |
| Cellular Localization | Cell membrane; Lipid-anchor; Cytoplasmic side |
Application
PHKA1 antibody detects PHKA1 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with PHKA1 antibody diluted by 1:500.
Application
PHKA1 antibody detects PHKA1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: PHKA1 protein stained by PHKA1 antibody diluted at 1:200. Blue: Hoechst 33342 staining.| Positive Control | 293T |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
