Application
Western blot analysis of PHLPP1 in SW480 cell lysate with PHLPP1 antibody at (A) 1 and (B) 2 ug/mL.Application
Immunocytochemistry of PHLPP1 in SW480 cells with PHLPP1 antibody at 2.5 ug/ml.Application
Immunofluorescence of PHLPP1 in SW480 cells with PHLPP1 antibody at 5 ug/ml.| Product Name | PHLPP1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PHLPP1This gene encodes a member of the serine/threonine phosphatase family. The encoded protein promotes apoptosis by dephosphorylating and inactivating the serine/threonine kinase Akt, and functions as a tumor suppressor in multiple types of cancer. Increased expression of this gene may also play a role in obesity and type 2 diabetes by interfering with Akt-mediated insulin signaling. [provided by RefSeq, Dec 2011] |
| Immunogen | PHLPP1 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human PHLPP1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFWB |
| WB: 1 - 2 ug/ml: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | SCOP PLEKHE1 PH domain and leucine rich repeat protein phosphatase 1 PHLPP |
|---|---|
| Target | PHLPP1 |
| Tissue Specificity | At least four isoforms are known to exist; this antibody will only detect the largest isoform. PHLPP1 antibody is predicted to not cross react with PHLPP2. |
Application
Western blot analysis of PHLPP1 in SW480 cell lysate with PHLPP1 antibody at (A) 1 and (B) 2 ug/mL.
Application
Immunocytochemistry of PHLPP1 in SW480 cells with PHLPP1 antibody at 2.5 ug/ml.
Application
Immunofluorescence of PHLPP1 in SW480 cells with PHLPP1 antibody at 5 ug/ml.| Application Notes | WB: 1 - 2 ug/ml: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Be stored at 4oC for three months and -20oC, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | PBS containing 0.02% sodium azide. |
