Application
Mouse tissue extract (50 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with Phospholamban (phospho Ser16) antibody diluted at 1:500.| Product Name | Phospholamban (phospho Ser16) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Phospholamban (phospho Ser16) (phospholamban)The protein encoded by this gene is found as a pentamer and is a major substrate for the cAMP-dependent protein kinase in cardiac muscle. The encoded protein is an inhibitor of cardiac muscle sarcoplasmic reticulum Ca(2+)-ATPase in the unphosphorylated state, but inhibition is relieved upon phosphorylation of the protein. The subsequent activation of the Ca(2+) pump leads to enhanced muscle relaxation rates, thereby contributing to the inotropic response elicited in heart by beta-agonists. The encoded protein is a key regulator of cardiac diastolic function. Mutations in this gene are a cause of inherited human dilated cardiomyopathy with refractory congestive heart failure. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Phospholamban (phospho Ser16). The exact sequence is proprietary. |
| Modification | p-Ser16 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Dog, Pig, Rabbit, Rhesus Monkey, BovineHuman (100%), Rat (100%), Dog (100%), Pig (100%), Rabbit (100%), Rhesus Monkey (100%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Target | Phospholamban (phospho Ser16) |
|---|---|
| Molecular Weight(MW) | 6 kDa (note) |
| Cellular Localization | Mitochondrion membrane; Single-pass membrane protein , Sarcoplasmic reticulum |
Application
Mouse tissue extract (50 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with Phospholamban (phospho Ser16) antibody diluted at 1:500.| Positive Control | Mouse heart |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
