Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using phospholamban antibody at 1:250 dilution.Application
Sample (50 µg of whole cell lysate) A: mouse heart 15% SDS PAGE diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | phospholamban antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to phospholamban (phospholamban)The protein encoded by this gene is found as a pentamer and is a major substrate for the cAMP-dependent protein kinase in cardiac muscle. The encoded protein is an inhibitor of cardiac muscle sarcoplasmic reticulum Ca(2+)-ATPase in the unphosphorylated state, but inhibition is relieved upon phosphorylation of the protein. The subsequent activation of the Ca(2+) pump leads to enhanced muscle relaxation rates, thereby contributing to the inotropic response elicited in heart by beta-agonists. The encoded protein is a key regulator of cardiac diastolic function. Mutations in this gene are a cause of inherited human dilated cardiomyopathy with refractory congestive heart failure. [provided by RefSeq] |
| Immunogen | Full length human phospholamban Recombinant protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Dog, Pig, Rabbit, Chicken, Rhesus Monkey, BovineRat (98%), Dog (96%), Pig (96%), Rabbit (98%), Chicken (82%), Rhesus Monkey (98%), Bovine (94%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CMD1P antibody PLB antibody PLN antibody cardiac phospholamban antibody phospholamban antibody |
|---|---|
| Molecular Weight(MW) | 6 kDa (note) |
| Cellular Localization | Mitochondrion membrane; Single-pass membrane protein , Sarcoplasmic reticulum |
Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using phospholamban antibody at 1:250 dilution.
Application
Sample (50 µg of whole cell lysate) A: mouse heart 15% SDS PAGE diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | mouse heart |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
