Application
Western blot analysis of mouse spleen extract probed with Left Lane: Vanadium treated Right lane: UntreatedApplication
Antibody Capture ELISA. Label: immobilized antigenApplication
Immunoblotting of fetal mouse brain extract (125 ug - A and 25 ug - B)| Product Name | Phosphothreonine antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to PhosphothreoninePhosphorylation of threonine residues is associated with many growth factors and oncogene protein kinases, and is important for cell signaling in activation, proliferation and differentiation. |
| Immunogen | Chemical / Small Molecule conjugated to KLH. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIPWB |
| ELISA(kinase assay): Use at 0.5 μg/mLWestern blot: Use at 4μg/mLIP: Use at 10 μg/250 μg protein sampleWill detect 100 ng of phosvitin in Western Blots and 0.5 ng of phosvitin with ELISA.Can be used for non-radioactive protein kinase assay (ELISA) using biotinylated peptide substrate and immunoblotting of abundant phosphoproteins. It is not recommended for immunoblotting of trace cellular phosphoproteins. Acetone precipitation of the protein extract followed by SDS denaturation is recommended for successful immunoprecipitation.: |
|
| Species Reactivity | Other |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Target | Phosphothreonine |
|---|---|
| Tissue Specificity | Reacts with free phosphothreonine but does not react with phosphoserine, threonine or phosphotyrosine. |
Application
Western blot analysis of mouse spleen extract probed with Left Lane: Vanadium treated Right lane: Untreated
Application
Antibody Capture ELISA. Label: immobilized antigen
Application
Immunoblotting of fetal mouse brain extract (125 ug - A and 25 ug - B)| Positive Control | Use mouse brain extract for immunoblotting. Use synthetic phosphopeptide (phosphorylated on threonine) for ELISA. |
|---|---|
| Application Notes | ELISA(kinase assay): Use at 0.5 μg/mLWestern blot: Use at 4μg/mLIP: Use at 10 μg/250 μg protein sampleWill detect 100 ng of phosvitin in Western Blots and 0.5 ng of phosvitin with ELISA.Can be used for non-radioactive protein kinase assay (ELISA) using biotinylated peptide substrate and immunoblotting of abundant phosphoproteins. It is not recommended for immunoblotting of trace cellular phosphoproteins. Acetone precipitation of the protein extract followed by SDS denaturation is recommended for successful immunoprecipitation.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | TBS with 0.01% sodium azide |
