Application
Immunocytochemistry of serum-starved A431 cells nontreated or treated with Calyculin A/Okadaic Acid, using Clone RM102 at 1/500 dilution (followed by PE conjugated secondary antibody, red) and DAPI (blue).Application
Western blot of serum-starved A431 cells nontreated or treated with Calyculin A/Okadaic Acid, using Clone RM102 at 1/2000 dilution.Application
Immunoprecipitation of Calyculin A/Okadaic Acid treated A431 cells by clone RM102 at 1/500 dilution, was then blotted with RM102. (1) whole lysate control; (2)IP by rabbit IgG control; (3) IP by RM111.Application
Rabbit monoclonal antibody clone RM102 recognizes Phosphorylated threonine in peptides with different sequences. It has minimum cross-reactivity with phosphorylated serine.| Product Name | Phosphothreonine antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Monoclonal antibody [RM102] to Phosphothreonine |
| Immunogen | Mixture of phosphothreonine-BSA conjugate and a phosphothreonine containing peptide |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | RM102 |
| Host Species | Rabbit |
| Tested Applications | ELISAFACSICC/IFIHCIPWB |
| FACS:1:200~1:500 ICC/IF:1:200~1:500 IHC:1:50~1:200 IP:1:100~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | All |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Target | Phosphothreonine |
|---|---|
| Tissue Specificity | This antibody reacts threonine-phosphorylated proteins. No cross reactivity with nonphosphorylated threonine, phosphoserine, and phosphotyrosine. It shows slight cross-reactivity with a few phospho-serine-containing peptides. |
Application
Immunocytochemistry of serum-starved A431 cells nontreated or treated with Calyculin A/Okadaic Acid, using Clone RM102 at 1/500 dilution (followed by PE conjugated secondary antibody, red) and DAPI (blue).
Application
Western blot of serum-starved A431 cells nontreated or treated with Calyculin A/Okadaic Acid, using Clone RM102 at 1/2000 dilution.
Application
Immunoprecipitation of Calyculin A/Okadaic Acid treated A431 cells by clone RM102 at 1/500 dilution, was then blotted with RM102. (1) whole lysate control; (2)IP by rabbit IgG control; (3) IP by RM111.
Application
Rabbit monoclonal antibody clone RM102 recognizes Phosphorylated threonine in peptides with different sequences. It has minimum cross-reactivity with phosphorylated serine.| Application Notes | FACS:1:200~1:500 ICC/IF:1:200~1:500 IHC:1:50~1:200 IP:1:100~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS with 50% Glycerol and 1% BSA. 0.09% Sodium azide was added as a preservative. |
