Application
Immunocytochemistry of serum-starved A431 cells nontreated or treated with EGF, using Clone RM111 at 1/500 dilution (followed by a PE conjugated secondary antibody, red) and DAPI (blue).Application
Western blot of serum-starved A431 cellsnontreated or treated with EGF, using Clone RM111 at 1/5000 dilution. The blot was exposed to films at different time points.Application
Immunoprecipitation of EGF-treated A431 cells by clone RM111 at 1/1000 dilution, was blotted with an anti-EGFR rabbit monoclonal antibody. (1) whole lysate control; (2) IP by rabbit IgG control; (3) IP by RM111Application
Luminex analysis of RM111 reaction to phosphorylated or nonphosphorylated Serine, Threonine, and Tyrosine. Clone RM111 only reacted to phosphorylated Tyrosine.| Product Name | Phosphotyrosine antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Monoclonal antibody [RM111] to Phosphotyrosine |
| Immunogen | Phosphotyrosine-BSA conjugate |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | RM111 |
| Host Species | Rabbit |
| Tested Applications | ELISAFACSICC/IFIHCIPWBMultiplexing |
| FACS:1:200~1:500 ICC/IF:1:200~1:500 IHC:1:50~1:200 IP:1:100~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | All |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Target | Phosphotyrosine |
|---|---|
| Tissue Specificity | This antibody reacts to tyrosine-phosphorylated proteins. No cross reactivity with nonphosphorylated tyrosine, phosphoserine, and phosphorthreonine |
Application
Immunocytochemistry of serum-starved A431 cells nontreated or treated with EGF, using Clone RM111 at 1/500 dilution (followed by a PE conjugated secondary antibody, red) and DAPI (blue).
Application
Western blot of serum-starved A431 cellsnontreated or treated with EGF, using Clone RM111 at 1/5000 dilution. The blot was exposed to films at different time points.
Application
Immunoprecipitation of EGF-treated A431 cells by clone RM111 at 1/1000 dilution, was blotted with an anti-EGFR rabbit monoclonal antibody. (1) whole lysate control; (2) IP by rabbit IgG control; (3) IP by RM111
Application
Luminex analysis of RM111 reaction to phosphorylated or nonphosphorylated Serine, Threonine, and Tyrosine. Clone RM111 only reacted to phosphorylated Tyrosine.| Application Notes | FACS:1:200~1:500 ICC/IF:1:200~1:500 IHC:1:50~1:200 IP:1:100~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS with 50% Glycerol and 1% BSA. 0.09% Sodium azide was added as a preservative. |
