Application
PHOX2B antibody detects PHOX2B protein at nucleus by immunofluorescent analysis. Sample: SK-N-AS cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PHOX2B protein stained by PHOX2B antibody diluted at 1:1000. Blue: Hoechst 33342 staining.Application
Sample(30 ug whole cell lysate) A:HeLa S3 12% SDS PAGE diluted at 1:1000| Product Name | PHOX2B antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to PHOX2B (paired-like homeobox 2b)The DNA-associated protein encoded by this gene is a member of the paired family of homeobox proteins localized to the nucleus. The protein functions as a transcription factor involved in the development of several major noradrenergic neuron populations and the determination of neurotransmitter phenotype. The gene product is linked to enhancement of second messenger-mediated activation of the dopamine beta-hydroylase, c-fos promoters and several enhancers, including cyclic amp-response element and serum-response element. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human PHOX2B. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Xenopus laevisMouse (100%), Xenopus laevis (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | NBLST2 antibody NBPhox antibody PMX2B antibody PHOX2B antibody neuroblastoma Phox antibody neuroblastoma paired-type homeobox protein antibody paired mesoderm homeobox 2b antibody paired mesoderm homeobox protein 2B antibody PHOX2B homeodomain prot |
|---|---|
| Target | PHOX2B |
| Molecular Weight(MW) | 32 kDa (note) |
| Cellular Localization | Nucleus |
Application
PHOX2B antibody detects PHOX2B protein at nucleus by immunofluorescent analysis. Sample: SK-N-AS cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PHOX2B protein stained by PHOX2B antibody diluted at 1:1000. Blue: Hoechst 33342 staining.
Application
Sample(30 ug whole cell lysate) A:HeLa S3 12% SDS PAGE diluted at 1:1000| Positive Control | 293T , A431 , HeLaS3 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
