Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary PI 4 Kinase type 2 beta antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. HC = hepatocarcinomaApplication
Western blot analysis of anti-PI4K II beta Pab in mouse brain (Lane A) and Saos-2 (Lane B) cell lysate. PI4K II beta (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.| Product Name | PI 4 Kinase 2 beta antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to PI 4 Kinase type 2 betaProtein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. |
| Immunogen | Synthetic peptide conjugated to KLH, corresponding to amino acids 319-336 of Human PI4K II beta . |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| ELISA: Use at a dilution of 1/1000. WB: Use at a dilution of 1/100 - 1/500. Detects a band of approximately 55 kDa (predicted molecular weight: 57 kDa). Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 0.2mg/ml |
| Purification | Protein G |
| Gene Synonyms | FLJ11105 PI4KIIB PIK42B |
|---|---|
| Target | PI 4 Kinase 2 beta |
| Cellular Localization | Cytoplasmic and Golgi Apparatus |
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary PI 4 Kinase type 2 beta antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. HC = hepatocarcinoma
Application
Western blot analysis of anti-PI4K II beta Pab in mouse brain (Lane A) and Saos-2 (Lane B) cell lysate. PI4K II beta (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.| Positive Control | Mouse brain and Saos 2 cell lysate |
|---|---|
| Application Notes | ELISA: Use at a dilution of 1/1000. WB: Use at a dilution of 1/100 - 1/500. Detects a band of approximately 55 kDa (predicted molecular weight: 57 kDa). Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.09% Sodium Azide; Constituents: PBS |
