Application
Immunohistochemical analysis of paraffin-embedded Human Breast Cancer, using PI3K gamma antibody.Application
Western Blot analysis of PIK3CG expression in transfected 293T cell line by PIK3CG polyclonal antibody. A: Non-transfected lysate. B: PIK3CG transfected lysate. 7.5% SDS PAGE diluted at 1:500Application
Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PI3 kinase p110 gamma antibody [C3], C-term diluted at 1:1000.Application
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PI3K gamma antibody [C3], C-term diluted at1:500.| Product Name | PI3 kinase p110 gamma antibody [C3], C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PI3 kinase p110 gamma (phosphoinositide-3-kinase, catalytic, gamma polypeptide)Phosphoinositide 3-kinases (PI3Ks) phosphorylate inositol lipids and are involved in the immune response. The protein encoded by this gene is a class I catalytic subunit of PI3K. Like other class I catalytic subunits (p110-alpha p110-beta, and p110-delta), the encoded protein binds a p85 regulatory subunit to form PI3K. This gene is located in a commonly deleted segment of chromosome 7 previously identified in myeloid leukemias. Several transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jun 2015] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PI3K gamma. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Pig, Rhesus MonkeyPig (100%), Rhesus Monkey (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | 1-phosphatidylinositol 3-kinase antibody, p110-gamma antibody, p120-PI3K antibody, phosphatidylinositol 3 kinase gamma, p110 gamma antibody, phosphatidylinositol 3-kinase catalytic 110-kD gamma antibody, phosphatidylinositol 3-kinase, catalytic, gamma |
|---|---|
| Molecular Weight(MW) | 126 kDa (note) (3) |
Application
Immunohistochemical analysis of paraffin-embedded Human Breast Cancer, using PI3K gamma antibody.
Application
Western Blot analysis of PIK3CG expression in transfected 293T cell line by PIK3CG polyclonal antibody. A: Non-transfected lysate. B: PIK3CG transfected lysate. 7.5% SDS PAGE diluted at 1:500
Application
Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PI3 kinase p110 gamma antibody [C3], C-term diluted at 1:1000.
Application
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PI3K gamma antibody [C3], C-term diluted at1:500.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
