Application
Untreated (–) and treated (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PI3 kinase p85 (phospho Tyr467)/p55 (phospho Tyr199) antibody diluted at 1:500.| Product Name | PI3 kinase p85 (phospho Tyr467)/p55 (phospho Tyr199) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PI3 kinase p85 (phospho Tyr467)/p55 (phospho Tyr199)Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in three transcript variants encoding different isoforms. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human PI3 kinase p85 (phospho Tyr467)/p55 (phospho Tyr199). The exact sequence is proprietary. |
| Modification | p-Tyr199 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:200~1:1000 *Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Zebrafish, Rhesus MonkeyMouse (100%), Rat (100%), Zebrafish (100%), Rhesus Monkey (100%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Target | PI3 kinase p85 (phospho Tyr467)/p55 (phospho Tyr199) |
|---|---|
| Molecular Weight(MW) | 84 kDa (note) (4) |
Application
Untreated (–) and treated (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PI3 kinase p85 (phospho Tyr467)/p55 (phospho Tyr199) antibody diluted at 1:500.| Application Notes | WB:1:200~1:1000 *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 50% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
