Application
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with PIK3R1-pY580 , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Western blot analysis of PIK3R1 (arrow) using rabbit polyclonal PIK3R1 Antibody (Y580) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PIK3R1 gene (Lane 2).| Product Name | PI3 kinase p85 alpha antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to PI3 kinase p85 alphaBinds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p11 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. |
| Immunogen | KLH conjugated synthetic peptide corresponding to amino acid residues surrounding Y580 of human PIK3R1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| ELISA: Use at 1:1,000 | |
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Phosphatidylinositol 3-kinase regulatory subunit alpha, PtdIns-3-kinase p85-alpha, PIK3R1, GRB1, Phosphatidylinositol3kinase regulatory subunit alpha, PI3-kinase p85-subunit alpha, PI3K, PI3kinase p85subunit alpha, PtdIns3kinase p85alpha, Phosphatidylinosi |
|---|---|
| Target | PIK3R1 |
| Molecular Weight(MW) | 83.598 kDa (note) |
Application
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with PIK3R1-pY580 , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Western blot analysis of PIK3R1 (arrow) using rabbit polyclonal PIK3R1 Antibody (Y580) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PIK3R1 gene (Lane 2).| Application Notes | ELISA: Use at 1:1,000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
