Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using PI3K C2 alpha antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:500.Application
PI3K C2 alpha antibody [C3], C-term detects PIK3C2A protein at cytosol on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma. PI3K C2 alpha antibody [C3], C-term dilution: 1:200.Application
Sample (30 µg of whole cell lysate) A: A431 5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | PI3K C2 alpha antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PI3 kinase C2 alpha (phosphoinositide-3-kinase, class 2, alpha polypeptide)The protein encoded by this gene belongs to the phosphoinositide 3-kinase (PI3K) family. PI3-kinases play roles in signaling pathways involved in cell proliferation, oncogenic transformation, cell survival, cell migration, and intracellular protein trafficking. This protein contains a lipid kinase catalytic domain as well as a C-terminal C2 domain, a characteristic of class II PI3-kinases. C2 domains act as calcium-dependent phospholipid binding motifs that mediate translocation of proteins to membranes, and may also mediate protein-protein interactions. The PI3-kinase activity of this protein is not sensitive to nanomolar levels of the inhibitor wortmanin. This protein was shown to be able to be activated by insulin and may be involved in integrin-dependent signaling. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PI3K C2 alpha. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, RatMouse (100%), Rat (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CPK antibody DKFZp686L193 antibody MGC142218 antibody PI3-K-C2(ALPHA) antibody PI3-K-C2A antibody PIK3C2A antibody ptdIns-3-kinase C2 subunit alpha antibody C2-containing phosphatidylinositol kinase antibody phosphatidylinositol-4-phosphate 3-kinas |
|---|---|
| Molecular Weight(MW) | 191 kDa (note) (3) |
| Cellular Localization | Cell membrane , Golgi apparatus , Cytoplasmic vesicle , clathrin-coated vesicle , Nucleus , Cytoplasm |
Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using PI3K C2 alpha antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:500.
Application
PI3K C2 alpha antibody [C3], C-term detects PIK3C2A protein at cytosol on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma. PI3K C2 alpha antibody [C3], C-term dilution: 1:200.
Application
Sample (30 µg of whole cell lysate) A: A431 5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | A431 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
