Application
Immunofluorescence analysis of methanol-fixed HeLa, using PI3K p110 beta antibody at 1:200 dilution.Application
Sample (50 ug of whole cell lysate) A: C2C12 B: C2C12+AEA10mM diluted at 1:1000Application
PI3K p110 beta antibody [N3C2], Internal detects PIK3CB protein by Western blot analysis. A. 30 μg PC-12 whole cell lysate/extract 5 % SDS-PAGE PI3K p110 beta antibody [N3C2], Internal dilution: 1:1000Application
Sample (30 ug of whole cell lysate) A: H1299 7.5% SDS PAGE diluted at 1:1000Application
PI3K p110 beta antibody [N3C2], Internal detects PI3K p110 beta protein at cytoplasm and nucleus in rat testis by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. PI3K p110 beta antibody [N3C2], Internal diluted at 1:400.| Product Name | PI3K p110 beta antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PI3 kinase p110 beta (phosphoinositide-3-kinase, catalytic, beta polypeptide)Phosphoinositide 3-kinases (PI3Ks) phosphorylate the 3-prime OH position of the inositol ring of inositol lipids. They have been implicated as participants in signaling pathways regulating cell growth by virtue of their activation in response to various mitogenic stimuli. PI3Ks are composed of a 110-kD catalytic subunit, such as PIK3CB, and an 85-kD adaptor subunit (Hu et al., 1993 [PubMed 8246984]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PI3K p110 beta. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Chicken, BovineChicken (91%), Bovine (96%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp779K1237 antibody MGC133043 antibody P110BETA antibody PI3K antibody PI3KBETA antibody PIK3C1 antibody PIK3CB antibody ptdIns-3-kinase subunit beta antibody PtdIns-3-kinase p110 antibody PI3K-beta antibody PI3-kinase p110 subunit beta antibo |
|---|---|
| Molecular Weight(MW) | 123 kDa (note) (2) |
Application
Immunofluorescence analysis of methanol-fixed HeLa, using PI3K p110 beta antibody at 1:200 dilution.
Application
Sample (50 ug of whole cell lysate) A: C2C12 B: C2C12+AEA10mM diluted at 1:1000
Application
PI3K p110 beta antibody [N3C2], Internal detects PIK3CB protein by Western blot analysis. A. 30 μg PC-12 whole cell lysate/extract 5 % SDS-PAGE PI3K p110 beta antibody [N3C2], Internal dilution: 1:1000
Application
Sample (30 ug of whole cell lysate) A: H1299 7.5% SDS PAGE diluted at 1:1000
Application
PI3K p110 beta antibody [N3C2], Internal detects PI3K p110 beta protein at cytoplasm and nucleus in rat testis by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. PI3K p110 beta antibody [N3C2], Internal diluted at 1:400.| Positive Control | H1299 , C2C12 , C2C12+AEA10mM , PC-12 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
