Application
Western blot analysis of extracts from 293 cells untreated or treated with sorbitol using PI3K P85 (phospho-Tyr467) Antibody .| Product Name | PI3K p85/p55 (phospho Tyr467/199) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PI3K P85 (Phospho Tyr467)Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain and regulates their kinase activity. During insulin stimulation, it also binds to IRS-1./Binds to activated (phosphorylated) protein-tyrosine kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 467 (L-Y(p)-E-E-Y) derived from Human PI3K P85. |
| Modification | p-Tyr467/199 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | GRB1, p85, p85-ALPHA, PIK3R1, phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha, phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha), PI3K regulatory subunit alpha, ptdIns-3-kinase regulatory subunit alpha, phosphatidyl |
|---|---|
| Target | PI3K P85 (Phospho Tyr467) |
| Molecular Weight(MW) | 85 (note) |
Application
Western blot analysis of extracts from 293 cells untreated or treated with sorbitol using PI3K P85 (phospho-Tyr467) Antibody .| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
