Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with PI3-Kinase p150 antibody diluted at 1:1000.Application
Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with PI3-Kinase p150 antibody diluted at 1:1000.Application
Mouse tissue extract (50 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with PI3-Kinase p150 antibody diluted at 1:1000.Application
Immunoprecipitation of PI3-Kinase p150 protein from HeLa whole cell extracts using 5 μg of PI3-Kinase p150 antibody . Western blot analysis was performed using PI3-Kinase p150 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
PI3-Kinase p150 antibody detects PI3-Kinase p150 protein at cytoplasm in mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. PI3-Kinase p150 antibody diluted at 1:500.| Product Name | PI3-Kinase p150 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PI3-Kinase p150 (phosphoinositide-3-kinase, regulatory subunit 4)Regulatory subunit of the PI3K complex. May regulate membrane trafficking late in the endocytic pathway. |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human PI3-Kinase p150. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, BovineRat (100%), Bovine (92%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PI3-Kinase p150 antibody , PI3-Kinase p150 antibody , PI3-kinase regulatory subunit 4 antibody , PIK3R4 antibody , VPS15 antibody , p150 antibody , phosphoinositide-3-kinase regulatory subunit 4 antibody |
|---|---|
| Target | PI3-Kinase p150 |
| Molecular Weight(MW) | 153 kDa (note) |
Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with PI3-Kinase p150 antibody diluted at 1:1000.
Application
Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with PI3-Kinase p150 antibody diluted at 1:1000.
Application
Mouse tissue extract (50 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with PI3-Kinase p150 antibody diluted at 1:1000.
Application
Immunoprecipitation of PI3-Kinase p150 protein from HeLa whole cell extracts using 5 μg of PI3-Kinase p150 antibody . Western blot analysis was performed using PI3-Kinase p150 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
PI3-Kinase p150 antibody detects PI3-Kinase p150 protein at cytoplasm in mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. PI3-Kinase p150 antibody diluted at 1:500.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
