Application
Immunohistochemical analysis of paraffin-embedded U87 xenograft, using PIAS2 antibody at 1:500 dilution.Application
Sample (30 µg of whole cell lysate) A: Jurkat B: Raji C: K562 D: THP-1 E: HL-60 F: NCI-H929 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (30 µg of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 7.5% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | PIAS2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PIAS2 (protein inhibitor of activated STAT, 2)This gene encodes a protein involved in the regulation of transcription factors involved in MAP kinase signaling. The symbol MIZ1 has also been associated with ZBTB17 which is a different gene located on chromosome 1. Two alternatively spliced transcripts encoding different isoforms have been described. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human PIAS2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Predicted Reactivity | RatRat (94%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MGC102682 antibody, MIZ1 antibody, PIASX antibody, PIASX-ALPHA antibody, PIASX-BETA antibody, SIZ2 antibody, ZMIZ4 antibody, miz antibody, PIAS2 antibody, protein inhibitor of activated STAT X antibody, DAB2-interacting protein antibody, E3 SUMO-protein li |
|---|---|
| Molecular Weight(MW) | 68 kDa (note) (6) |
| Cellular Localization | Nucleus speckle |
Application
Immunohistochemical analysis of paraffin-embedded U87 xenograft, using PIAS2 antibody at 1:500 dilution.
Application
Sample (30 µg of whole cell lysate) A: Jurkat B: Raji C: K562 D: THP-1 E: HL-60 F: NCI-H929 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (30 µg of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 7.5% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
