Application
Western blot analysis of PAIASg in A) human liver, B) mouse liver, C) rat liver and D) human Jurkat lysate at 5 ug/ml.| Product Name | PIASy antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PIASyThe interaction of a cytokine with its specific cell surface receptor triggers the activation of intracellular signaling pathways that ultimately program the cellular response. The signal transducers and activators of transcription (STAT) proteins play important roles in the activation of these pathways. Recently, a family of proteins has been identified that inhibits activated STATS and are named as Protein Inhibitors of Activated STAT (PIAS). PIAS proteins comprise a family of five mammalian proteins, which have been identified as potentially important down regulators of the STAT-signaling pathway. The cDNA for mouse and human PIASg encodes a protein of 507 amino acids that is highly homologous to the human protein and is expressed in the mouse as early as d 7.5 of gestation. In situ hybridizations of staged mouse embryos localized the transcript for the PIAS gamma gene to the limbs, neuroepithelium, and the inner root sheath of the hair follicle, suggesting a role in the development of these structures. This protein is also present in hair follicle of human scalp hair and in monkey neuronal cells. Thus PIASg exhibits a highly selective pattern of expression, suggesting that it modulates the response of cells to developmental cues. |
| Immunogen | Synthetic peptides QLVQFDCSPELFKKIKE and KAVQVVLRICYS, corresponding to amino acids 44-60 and 187-198 of Human PAIAS gamma. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: Use at a concentration of 1 - 2 μg/ml. Detects a band of approximately 55 kDa. Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Protein G |
| Gene Synonyms | FLJ12419, MGC35296, PIASY, Piasg, ZMIZ6 |
|---|---|
| Target | PIASy |
| Cellular Localization | Nuclear |
Application
Western blot analysis of PAIASg in A) human liver, B) mouse liver, C) rat liver and D) human Jurkat lysate at 5 ug/ml.| Application Notes | WB: Use at a concentration of 1 - 2 μg/ml. Detects a band of approximately 55 kDa. Optimal dilutions/concentrations should be determined by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.02% Sodium Azide; Constituents: 0.2% Gelatin, PBS |
