Application
PIASy antibody detects PIASy protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg whole cell lysate/extract of human PIAS4-transfected 293T cells 10 % SDS-PAGE PIASy antibody dilution: 1:5000| Product Name | PIASy antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PIASy (protein inhibitor of activated STAT, 4)Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway, the Wnt pathway and the steroid hormone signaling pathway. Involved in gene silencing. Promotes PARK7 sumoylation. In Wnt signaling, represses LEF1 and enhances TCF4 transcriptional activities through promoting their sumoylations. |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human PIASy. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Chimpanzee, BovineMouse (100%), Rat (100%), Chimpanzee (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | protein inhibitor of activated STAT, 4 , Piasg , ZMIZ6 , PIAS4 , PIASY |
|---|---|
| Target | PIASy |
| Molecular Weight(MW) | 57 kDa (note) |
| Cellular Localization | Nucleus |
Application
PIASy antibody detects PIASy protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg whole cell lysate/extract of human PIAS4-transfected 293T cells 10 % SDS-PAGE PIASy antibody dilution: 1:5000| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
