| Product Name | PICK1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to PICK1PSD-95/DLG/ZO-1 (PDZ) domain-containing proteins play a central role in synaptic membrane protein localization. The protein interacting with protein kinase C (PICK 1) is a synaptic PDZ domain protein that also contains a coiled-coil and acidic domain. Studies indicate that PICK 1 functions as a targeting and transport protein and has been shown to interact with protein kinase C alpha (PKC alpha), AMPA-type glutamate receptors, and several other membrane receptors via its PDZ domain. The interaction of PICK 1 with PKC alpha is highly dependent on the activation of the kinase. It appears that PICK 1 directs the activated form of PKC alpha to membrane anchored GluR2. Once phosphorylated by PKC alpha, GluR2 is released from the synaptic anchor proteins. Once released, GluR2 is transported from the synaptic membrane in a PICK 1-dependent manner. |
| Immunogen | 6xhis tagged fusion protein: EKMELLDQKH VQDIVFQLQR FVSTMSKYYN DCYAVLQDAD VFPIEVDLAH TTLAYGPNQG SFTDGEEEDE EEEDGAAREV SKDACGATGP TDKGGSWCDS, corresponding to C terminal amino acids 317-416 of Mouse PICK1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: Use at a dilution of 1/500 - 1/1000. Predicted molecular weight: 50 kDa. Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Prkcabp |
|---|---|
| Target | PICK1 |
| Tissue Specificity | Recognizes PICK1. |
| Cellular Localization | Cytoplasmic; concentrated in the perinuclear region. Also present at excitatory synapses. |
| Application Notes | WB: Use at a dilution of 1/500 - 1/1000. Predicted molecular weight: 50 kDa. Optimal dilutions/concentrations should be determined by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.05% Sodium Azide; Constituents: 5mg/ml BSA, 0.15M Sodium Chloride, 50mM Glycine, 0.2mM Tris, 0.1mM EDTA. pH 7.4 |
