Application
PIGR antibody [N2C1], Internal detects PIGR protein at cell membrane in human lung adenocarcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung adenocarcinoma. PIGR antibody [N2C1], Internal diluted at 1:500.Application
Sample (30 µg of whole cell lysate) A: Molt-4 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunofluorescence analysis of methanol-fixed HepG2, using PIGR antibody at 1:200 dilution.Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft , using PIGR antibody at 1:500 dilution.| Product Name | PIGR antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to PIGR (polymeric immunoglobulin receptor)The poly-Ig receptor is expressed on several glandular epithelia including those of liver and breast. It mediates transcellular transport of polymeric immunoglobulin molecules. It is a member of the immunoglobulin superfamily (Hood et al., 1985 [PubMed 3917857]). The receptor has 5 units with homology to the variable (V) units of immunoglobulins and a transmembrane region, which also has some homology to certain immunoglobulin variable regions.[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PIGR. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FLJ22667 antibody, MGC125361 antibody, MGC125362 antibody, PIGR antibody, polymeric immunoglobulin receptor antibody, hepatocellular carcinoma associated protein TB6 antibody, hepatocellular carcinoma-associated protein TB6 antibody, poly-Ig receptor antib |
|---|---|
| Target | PIGR |
| Molecular Weight(MW) | 83 kDa (note) |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein , Secretory component: Secreted |
Application
PIGR antibody [N2C1], Internal detects PIGR protein at cell membrane in human lung adenocarcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung adenocarcinoma. PIGR antibody [N2C1], Internal diluted at 1:500.
Application
Sample (30 µg of whole cell lysate) A: Molt-4 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunofluorescence analysis of methanol-fixed HepG2, using PIGR antibody at 1:200 dilution.
Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft , using PIGR antibody at 1:500 dilution.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
