Western blot analysis of extracts from 293/COLO/HuvEc/Jurkat cells, using PIGX Antibody. The lane on the right is treated with the synthesized peptide.
Rabbit polyclonal antibody to PIGXEssential component of glycosylphosphatidylinositol-mannosyltransferase 1 which transfers the first of the 4 mannoses in the GPI-anchor precursors during GPI-anchor biosynthesis. Probably acts by stabilizing the mannosyltransferase PIGM.
Immunogen
The antiserum was produced against synthesized peptide derived from human PIGX.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
ELISA: 1:40000
Optimal dilutions should be determined experimentally by the end user.
Species Reactivity
Human
Concentration
1mg/ml
Purification
Affinity purified
Target Information
Gene Synonyms
PIG-X, PIGX, Phosphatidylinositol-glycan biosynthesis class X protein, Phosphatidylinositolglycan biosynthesis class X protein, PIG X
Target
PIGX
Molecular Weight(MW)
28 kDa (note)
Tissue Specificity
PIGX Antibody detects endogenous levels of total PIGX protein.
Application
Application
Western blot analysis of extracts from 293/COLO/HuvEc/Jurkat cells, using PIGX Antibody. The lane on the right is treated with the synthesized peptide.
Application Notes
ELISA: 1:40000
Optimal dilutions should be determined experimentally by the end user.
Additional Information
Form
Liquid
Storage Instructions
Store at -20 degrees Celcius. Product is stable for at least 1 year.
Storage Buffer
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.