Rabbit polyclonal antibody to PIG-YGlycosylphosphatidylinositol (GPI) lipid anchoring is an important post-translational modification of proteins that takes place in the endoplasmic reticulum. The synthesis of GPI is initiated by GPI-N-acetylglucosaminyltransferase (GPI-GnT), a complex of proteins including PIG-A, PIG-H, PIG-C, GPI1, and DPM2. PIG-Y, the mammalian homolog to yeast Eri1p, is also thought to be involved in the biosynthesis of GPI. The PIG-Y gene encodes two proteins, one of which arises from leaky scanning of the mRNA. This antibody only detects isoform 1 of PIG-Y. Despite its predicted molecular weight, PIG-Y often migrates at 28-30 kDa in SDS-PAGE.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAIHC-PWB
Species Reactivity
HumanMouse
Concentration
1mg/ml
Purification
Affinity purified
Target Information
Gene Synonyms
Phosphatidylinositol glycan anchor biosynthesis class Y, PIG-Y, PIGY, PreY, PIG Y
Target
PIG-Y
Tissue Specificity
This antibody is specific for PIG-Y C-Terminus
Application
Application
Immunohistochemistry of PIGY in human spleen tissue with PIGY antibody at 2.5 ug/ml.
Application
Western blot analysis of PIG-Y in A-20 cell lysate with PIG-Y antibody at 2 ug/ml.
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.