Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PIK3AP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PIK3AP1.Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PIK3AP1 monoclonal antibody.Application
Flow cytometric Analysis of Jurkat cells, using anti-PIK3AP1 antibody,(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).Application
HEK293T cells transfected with either RC214125 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PIK3AP1 antibody, and then analyzed by flow cytometry.| Product Name | PIK3AP1 antibody [6H6] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [6H6] to PIK3AP1Involved in the activation of phosphoinositide 3-kinase (PI3K) in B-cells and in natural killer (NK) cells. Couples B-cell antigen receptor (BCR) to PI3K activation by providing a docking site for the PI3K subunit PIK3R1, which contributes to B-cell development. Seems to have a complementary role with CD19 in PI3K activation (By similarity). May be involved in the survival of mature B cells via activation of REL. |
| Immunogen | Full length human recombinant protein of human PIK3AP1 (NP_689552) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 6H6 |
| Host Species | Mouse |
| Tested Applications | FACSWB |
| ICC/IF 1:100 | |
| Species Reactivity | HumanMonkeyRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | BCAP, RP11-34E5.3, RP1134E5.3, PIK3AP1 |
|---|---|
| Target | PIK3AP1 |
| Tissue Specificity | is specific for human PIK3AP1 (NP_689552). |
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PIK3AP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PIK3AP1.
Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PIK3AP1 monoclonal antibody.
Application
Flow cytometric Analysis of Jurkat cells, using anti-PIK3AP1 antibody,(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Application
HEK293T cells transfected with either RC214125 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PIK3AP1 antibody, and then analyzed by flow cytometry.| Application Notes | ICC/IF 1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
