Application
PIK4CB Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with PIK4CB Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Western blot analysis of PIK4CB Antibody (Center) in Hela cell line lysates (35ug/lane). PIK4CB (arrow) was detected using the purified Pab.| Product Name | PIK4CB antibody, Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to PIK4CB (Internal)Phosphorylates phosphatidylinositol (PI) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate (PIP). May regulate Golgi disintegration/reorganization during mitosis, possibly via its phosphorylation. Involved in Golgi-to-plasma membrane trafficking. |
| Immunogen | KLH conjugated synthetic peptide between 526~556 amino acids from the Center region of human PIK4CB. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| ELISA: Use at 1:1,000 | |
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Unpurified |
| Gene Synonyms | PI4K-beta, PIK4CB, PI4K92, Phosphatidylinositol4kinase beta, PtdIns 4-kinase beta, NPIK, PtdIns4kinase beta, PtdIns 4kinase beta, PI4KB, Phosphatidylinositol 4-kinase beta, PI4Kbeta, Phosphatidylinositol 4kinase beta, PI4K beta, PtdIns 4 kinase beta |
|---|---|
| Target | PIK4CB |
| Molecular Weight(MW) | 91.379 kDa (note) |
| Cellular Localization | Found in the outer membrane of mitochondria and membranes of the rough endoplasmic reticulum. Recruited to the Golgi complex by the small GTPase ARF to stimulate the synthesis of phosphatidylinositol 4,5-biphosphate (PIP2) on the Golgi complex. |
Application
PIK4CB Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with PIK4CB Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Western blot analysis of PIK4CB Antibody (Center) in Hela cell line lysates (35ug/lane). PIK4CB (arrow) was detected using the purified Pab.| Application Notes | ELISA: Use at 1:1,000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
