Application
Western blot analysis of PIM1 using Jurkat cell lysate| Product Name | PIM1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to PIM1The proto-oncogene Pim1 belongs to a family of serine/threonine protein kinases that are highly conserved through evolution in multicellular organisms. Pim1 is involved in the control of cytokine-mediated cell proliferation, differentiation and survival of lymphoid and myeloid cells as well as others. Expression of Pim1 can be stimulated by a variety of growth factors and is regulated at four different levels: transcriptional, post-transcriptional, translational and post-translational. Recently, Pim1 has been shown to enhance the activities of p100, c-Myb and Cdc 25a and in part this might explain reported effects of Pim1 on mitogenesis. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| Western blot analysis (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody recognizes ~33 kDa of PIM1 isoform from samples of human, mouse and rat origins. Reactivity to other species has not been determined. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PIM, Proviral Integration Site 1, Proviral Integration Site1, PIM1, PIM-1, PIM 1 |
|---|---|
| Target | PIM1 |
| Tissue Specificity | This antibody is specific for PIM1. |
Application
Western blot analysis of PIM1 using Jurkat cell lysate| Application Notes | Western blot analysis (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody recognizes ~33 kDa of PIM1 isoform from samples of human, mouse and rat origins. Reactivity to other species has not been determined. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | The antibody solution should be gently mixed before use. Stored at -20 °C for future use. |
| Storage Buffer | phosphate (PBS, pH 7.2) containing 30% glycerol, 0.5 % BSA and 0.01% thimerosal |
