Application
Western blot analysis of PIN1 (pS16) expression in HeLa insulin-treated (A); K562 (B); NIH3T3 (C); Raw264.7 insulin-treated (D); PC12 insulin-treated (E); C6 calyculin A-treated (F) whole cell lysates.| Product Name | PIN1 (phospho Ser16) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PIN1 (phospho Ser16)Peptidyl-prolyl cis/trans isomerases (PPIases) catalyze the cis/trans isomerization of peptidyl-prolyl peptide bonds. This gene encodes one of the PPIases, which specifically binds to phosphorylated ser/thr-pro motifs to catalytically regulate the post-phosphorylation conformation of its substrates. The conformational regulation catalyzed by this PPIase has a profound impact on key proteins involved in the regulation of cell growth, genotoxic and other stress responses, the immune response, induction and maintenance of pluripotency, germ cell development, neuronal differentiation, and survival. This enzyme also plays a key role in the pathogenesis of Alzheimer's disease and many cancers. Multiple alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Jun 2011] |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human PIN1. The exact sequence is proprietary. |
| Modification | p-Ser16 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: 1:500 - 1:1000 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PIN1 , DOD , peptidylprolyl cis/trans isomerase, NIMA-interacting 1 , UBL5 |
|---|---|
| Target | PIN1 (phospho Ser16) |
| Tissue Specificity | Recognizes endogenous levels of PIN1 (pS16) protein. |
Application
Western blot analysis of PIN1 (pS16) expression in HeLa insulin-treated (A); K562 (B); NIH3T3 (C); Raw264.7 insulin-treated (D); PC12 insulin-treated (E); C6 calyculin A-treated (F) whole cell lysates.| Application Notes | WB: 1:500 - 1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4 °C. Upon delivery aliquot and store at -20 °C for one year. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in 0.42% Potassium phosphate; 0.87% Sodium chloride; pH 7.3; 30% glycerol; and 0.01% sodium azide. |
