Application
Western blot analysis of PIP5K1A (arrow) using rabbit polyclonal hPIP5K1A-R34 . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PIP5K1A gene.Application
Western blot analysis of anti-PIP5K1A Pab in HeLa cell lysate. PIP5K1A (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.| Product Name | PIP5K1A antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to PIP5K1AProtein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. |
| Immunogen | Synthetic peptide conjugated to KLH, corresponding to amino acids 34-50 of Human PIP5K1 A. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| ELISA: Use at a dilution of 1/1000. WB: Use at a dilution of 1/100 - 1/500. Detects a band of approximately 61 kDa (predicted molecular weight: 65 kDa). Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Protein G |
| Target | PIP5K1 alpha |
|---|
Application
Western blot analysis of PIP5K1A (arrow) using rabbit polyclonal hPIP5K1A-R34 . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PIP5K1A gene.
Application
Western blot analysis of anti-PIP5K1A Pab in HeLa cell lysate. PIP5K1A (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.| Application Notes | ELISA: Use at a dilution of 1/1000. WB: Use at a dilution of 1/100 - 1/500. Detects a band of approximately 61 kDa (predicted molecular weight: 65 kDa). Optimal dilutions/concentrations should be determined by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.09% Sodium Azide; Constituents: PBS |
