Application
Pirin antibody detects PIR protein by Western blot analysis. A. 50 µg mouse liver lysate/extract 12 % SDS-PAGE Pirin antibody dilution: 1:1000Application
Pirin antibody detects Pirin protein at cytosol and nucleus on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. Pirin antibody dilution: 1:500.Application
Pirin antibody detects Pirin protein at cytosol and nucleus on rat brain stem by immunohistochemical analysis. Sample: Paraffin-embedded rat brain stem. Pirin antibody dilution: 1:500.Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Pirin antibody at 1:500 dilution.Application
Immunohistochemical analysis of paraffin-embedded MPG xenograft, using Pirin antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: Hela 12% SDS PAGE Pirin antibody diluted at 1:1000| Product Name | Pirin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Pirin (pirin (iron-binding nuclear protein))This gene encodes a member of the cupin superfamily. The encoded protein is an Fe(II)-containing nuclear protein expressed in all tissues of the body and concentrated within dot-like subnuclear structures. Interactions with nuclear factor I/CCAAT box transcription factor as well as B cell lymphoma 3-encoded oncoprotein suggest the encoded protein may act as a transcriptional cofactor and be involved in the regulation of DNA transcription and replication. Alternatively spliced transcript variants have been described. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Pirin. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Rhesus Monkey, BovineRhesus Monkey (98%), Bovine (90%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PIR antibody, probable quercetinase antibody, probable quercetin 2,3-dioxygenase PIR antibody, pirin antibody, pirin (iron-binding nuclear protein) antibody |
|---|---|
| Molecular Weight(MW) | 32 kDa (note) (3) |
| Cellular Localization | Nucleus |
Application
Pirin antibody detects PIR protein by Western blot analysis. A. 50 µg mouse liver lysate/extract 12 % SDS-PAGE Pirin antibody dilution: 1:1000
Application
Pirin antibody detects Pirin protein at cytosol and nucleus on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. Pirin antibody dilution: 1:500.
Application
Pirin antibody detects Pirin protein at cytosol and nucleus on rat brain stem by immunohistochemical analysis. Sample: Paraffin-embedded rat brain stem. Pirin antibody dilution: 1:500.
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Pirin antibody at 1:500 dilution.
Application
Immunohistochemical analysis of paraffin-embedded MPG xenograft, using Pirin antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: Hela 12% SDS PAGE Pirin antibody diluted at 1:1000| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
