Application
PITX1 Antibody (Center) flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
PITX1 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded human Lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PITX1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
Western blot analysis of PITX1 antibody (Center) in NCI-H460 cell line lysates (35ug/lane). PITX1 (arrow) was detected using the purified Pab.Application
Western blot analysis of PITX1 (arrow) using rabbit polyclonal PITX1 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PITX1 gene.| Product Name | PITX1 antibody, Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to PITX1 (Internal)May play a role in the development of anterior structures, and in particular, the brain and facies and in specifying the identity or structure of hindlimb. |
| Immunogen | KLH conjugated synthetic peptide between 129~159 amino acids from the Center region of human PITX1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| ELISA: Use at 1:1,000 | |
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Unpurified |
| Gene Synonyms | Hindlimb-expressed homeobox protein backfoot, Pairedlike homeodomain transcription factor1, Pairedlike homeodomain transcription factor 1, Paired-like homeodomain transcription factor 1, Homeobox protein PITX1, Hindlimbexpressed homeobox protein backfoot, |
|---|---|
| Target | PITX1 |
| Molecular Weight(MW) | 34.128 kDa (note) |
Application
PITX1 Antibody (Center) flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
PITX1 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded human Lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PITX1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Western blot analysis of PITX1 antibody (Center) in NCI-H460 cell line lysates (35ug/lane). PITX1 (arrow) was detected using the purified Pab.
Application
Western blot analysis of PITX1 (arrow) using rabbit polyclonal PITX1 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PITX1 gene.| Application Notes | ELISA: Use at 1:1,000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
