Application
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PIWIL4 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.Application
Immunohistochemical analysis of paraffin-embedded 59T xenograft, using PIWIL4 antibody at 1:500 dilution.Application
Immunofluorescence analysis of paraformaldehyde-fixed MCF-7, using PIWIL4 antibody at 1:200 dilution.| Product Name | PIWIL4 antibody [C2C3], C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PIWIL4 (piwi-like 4 (Drosophila))PIWIL4 belongs to the Argonaute family of proteins, which function in development and maintenance of germline stem cells (Sasaki et al., 2003 [PubMed 12906857]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human PIWIL4. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Rat, Pig, Rhesus MonkeyRat (80%), Pig (85%), Rhesus Monkey (98%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp686P01248 antibody, FLJ36156 antibody, HIWI2 antibody, MIWI2 antibody, PIWIL4 antibody, piwi-like protein 4 antibody, piwi-like 4 (Drosophila) antibody |
|---|---|
| Molecular Weight(MW) | 97 kDa (note) (2) |
| Cellular Localization | Nucleus (By similarity) , Cytoplasm (By similarity) |
Application
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PIWIL4 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Application
Immunohistochemical analysis of paraffin-embedded 59T xenograft, using PIWIL4 antibody at 1:500 dilution.
Application
Immunofluorescence analysis of paraformaldehyde-fixed MCF-7, using PIWIL4 antibody at 1:200 dilution.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
