Application
Western blot analysis of extracts of various cell lines using PRKAR1A antibody.Application
Immunohistochemistry of paraffin-embedded human oophoroma using PRKAR1A antibody at dilution of 1:100 (400x lens).Application
Immunofluorescence analysis of HeLa cell using PRKAR1A antibody. Blue: DAPI for nuclear staining.| Product Name | PKA I alpha reg antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PKA I alpha regcAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. This gene encodes one of the regulatory subunits. This protein was found to be a tissue-specific extinguisher that down-regulates the expression of seven liver genes in hepatoma x fibroblast hybrids. Mutations in this gene cause Carney complex (CNC). This gene can fuse to the RET protooncogene by gene rearrangement and form the thyroid tumor-specific chimeric oncogene known as PTC2. A nonconventional nuclear localiz |
| Immunogen | Recombinant protein of human PRKAR1A |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIHC-PWB |
| WB 1:500 - 1:2000, IHC 1:50 - 1:200, IF 1:50 - 1:200 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PKR1 , CNC1 , PPNAD1 , PRKAR1 , protein kinase, cAMP-dependent, regulatory, type I, alpha , TSE1 , CAR , ACRDYS1 , ADOHR , CNC |
|---|---|
| Target | PRKAR1A |
| Molecular Weight(MW) | 43 kDa (note) |
Application
Western blot analysis of extracts of various cell lines using PRKAR1A antibody.
Application
Immunohistochemistry of paraffin-embedded human oophoroma using PRKAR1A antibody at dilution of 1:100 (400x lens).
Application
Immunofluorescence analysis of HeLa cell using PRKAR1A antibody. Blue: DAPI for nuclear staining.| Application Notes | WB 1:500 - 1:2000, IHC 1:50 - 1:200, IF 1:50 - 1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
