Application
WB analysis of (1) untreated and (2) Alkaline Phosphatase-treated K562 lysates using PKA R2 (phospho Ser99) antibody [E151] at a dilution of 1:2500.Application
ICC/IF analysis of (A) untreated and (B) Alkaline Phosphatase treated HeLa cells using PKA R2 (phospho Ser99) antibody [E151] at a dilution of 1:500.Application
IHC-P analysis of (A) untreated and (B) Phosphatase treated lymph node metastatic tissues using PKA R2 (phospho Ser99) antibody [E151] at a dilution of 1:250.| Product Name | PKA R2 (phospho Ser99) antibody [E151] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit monoclonal antibody [E151] to PKA R2 (phospho Serine99)cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. It may interact with various A-kinase anchoring proteins and determine the subcellular localization of cAMP-dependent protein kinase. This subunit has been shown to regulate protein transport from endosomes to the Golgi apparatus and further to the endoplasmic reticulum (ER). [provided by RefSeq, Jul 2008] |
| Immunogen | A synthetic phospho-peptide corresponding to residues surrounding Ser99 of human PKA R2 |
| Modification | p-Ser99 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | E151 |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| WB: 1:2500, IHC-P: 1:250, IP: 1:20, ICC/IF: 1:500. The application notes include recommended starting dilutions optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | HumanMousePigRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Synonyms | PKR2 , PRKAR2A , PRKAR2 , protein kinase, cAMP-dependent, regulatory, type II, alpha |
|---|---|
| Target | PKA R2 (phospho Serine99) |
| Molecular Weight(MW) | 45 kDa (note) |
| Tissue Specificity | The antibody only detects PKA R2 phosphorylated on Serine 99. Phosphoserine 99 in Human corresponds to phosphoserine 96 in mouse. |
Application
WB analysis of (1) untreated and (2) Alkaline Phosphatase-treated K562 lysates using PKA R2 (phospho Ser99) antibody [E151] at a dilution of 1:2500.
Application
ICC/IF analysis of (A) untreated and (B) Alkaline Phosphatase treated HeLa cells using PKA R2 (phospho Ser99) antibody [E151] at a dilution of 1:500.
Application
IHC-P analysis of (A) untreated and (B) Phosphatase treated lymph node metastatic tissues using PKA R2 (phospho Ser99) antibody [E151] at a dilution of 1:250.| Application Notes | WB: 1:2500, IHC-P: 1:250, IP: 1:20, ICC/IF: 1:500. The application notes include recommended starting dilutions optimal dilutions/concentrations should be determined by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. |
| Storage Buffer | PBS containing 50% Glycerol, 0.05% BSA and 0.01% Sodium azide. |
