Application
PKC alpha antibody detects PKC alpha protein in the rod bipolar cells by immunohistochemical analysis. Sample: Frozen sectioned adult mouse retina. Green: PKC alpha protein stained by PKC alpha antibody diluted at 1:250. Blue: Fluoroshield with DAPI .Application
PKC alpha antibody detects PKC alpha protein by immunohistochemical analysis. Sample: Frozen sectioned adult mouse retina. Red: PKC alpha protein stained by PKC alpha antibody diluted at 1:250. Blue: Fluoroshield with DAPI .Application
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC alpha antibody diluted at 1:1000.Application
Various tissue extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC alpha antibody diluted at 1:2000.Application
PKC alpha antibody detects PKC alpha protein at cell membrane and cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PKC alpha protein stained by PKC alpha antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Product Name | PKC alpha antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PKC alpha (protein kinase C, alpha)Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PKC alpha. The exact sequence is proprietary |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-FWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Rabbit, Chicken, Rhesus Monkey, BovineRabbit (100%), Chicken (100%), Rhesus Monkey (100%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | protein kinase C, alpha , PRKCA , AAG6 , PKC-alpha , PKCA , PRKACA |
|---|---|
| Target | PKC alpha |
| Molecular Weight(MW) | 77 kDa (note) |
Application
PKC alpha antibody detects PKC alpha protein in the rod bipolar cells by immunohistochemical analysis. Sample: Frozen sectioned adult mouse retina. Green: PKC alpha protein stained by PKC alpha antibody diluted at 1:250. Blue: Fluoroshield with DAPI .
Application
PKC alpha antibody detects PKC alpha protein by immunohistochemical analysis. Sample: Frozen sectioned adult mouse retina. Red: PKC alpha protein stained by PKC alpha antibody diluted at 1:250. Blue: Fluoroshield with DAPI .
Application
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC alpha antibody diluted at 1:1000.
Application
Various tissue extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC alpha antibody diluted at 1:2000.
Application
PKC alpha antibody detects PKC alpha protein at cell membrane and cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PKC alpha protein stained by PKC alpha antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
