Application
Peptide Competition and Phosphatase Treatment: Lysates prepared from K562 cells stimulated with PMA were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were either treated with lambda phosphatase (1) or, left untreated (2-5), blocked with a 5% BSA-TBST buffer for two hours at room temperature, and incubated with primary antibody for two hours at room temperature in a 3% BSATBST buffer, following prior incubation with: no peptide (1, 2), the nonphosphopeptide corresponding to the immunogen (3), a generic phosphothreoninecontaining peptide (4), or, the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab)2 anti-rabbit IgG HRP conjugated and bands were detected. The data show that the peptide corresponding to PKC beta2 [pS641] blocks the antibody signal. The data also show that phosphatase stripping eliminates the signal, verifying that the antibody is phospho-specific.| Product Name | PKC beta 2 (phospho Thr641) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to PKC beta 2 ( phospho T641 )Protein Kinase C beta 2 (PKC beta 2) is an 80 kDa member of the conventional group (cPKCs: sensitive to diacylglycerol, phosphotidylserine and phorbol esters) of the PKC family of serine/threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival, transcriptional regulation and tumor promotion. PKC beta 2 is phosphorylated on three sites, threonine 500 in the activation loop, threonine 641 in the turn loop and serine 660 in the hydrophobic loop. Threonine 641 mediates PKC beta 2 binding to Hsp70 which regulates its stability and phosphorylation. Phosphorylation of threonine 641 is also crucial for PKC beta 2 proper subcellular localization and catalytic function as well as for its autophosphorylation on serine 660. |
| Immunogen | Synthetic phosphopeptide derived from a region of human PKC beta 2 that contains threonine 641. |
| Modification | p-Thr641 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: Use at a dilution of 1/1000. Detects a band of approximately 80 kDa. Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MGC41878, PKC-beta, PKCB, PRKCB1, PRKCB2, PKC beta, PKCbeta, PKC beta 2 (phospho T641), PKC beta 2 (phospho Thr641), PKC beta 2 (pT641), PKC beta 2 (pThr641), PKC beta 2 phospho T641, PKC beta 2 phospho Thr641, Phospho PKC beta 2 (T641), Phospho PKC beta 2 |
|---|---|
| Target | PKC beta 2 |
| Cellular Localization | Cytoplasmic and membrane associated. |
Application
Peptide Competition and Phosphatase Treatment: Lysates prepared from K562 cells stimulated with PMA were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were either treated with lambda phosphatase (1) or, left untreated (2-5), blocked with a 5% BSA-TBST buffer for two hours at room temperature, and incubated with primary antibody for two hours at room temperature in a 3% BSATBST buffer, following prior incubation with: no peptide (1, 2), the nonphosphopeptide corresponding to the immunogen (3), a generic phosphothreoninecontaining peptide (4), or, the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab)2 anti-rabbit IgG HRP conjugated and bands were detected. The data show that the peptide corresponding to PKC beta2 [pS641] blocks the antibody signal. The data also show that phosphatase stripping eliminates the signal, verifying that the antibody is phospho-specific.| Application Notes | WB: Use at a dilution of 1/1000. Detects a band of approximately 80 kDa. Optimal dilutions/concentrations should be determined by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.05% Sodium Azide; Constituents: 50% Glycerol, PBS, 1mg/ml BSA. pH 7.3 |
