Application
C. Immunofluorescent staining of HeLa cells using anti-PKC beta II RabMAb (catalog #).Application
B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti- PKC beta II RabMAb (catalog #).Application
A. Western blot analysis on K562 cell lysate using anti-PKC beta II RabMAb (catalog #) dilution 1:500| Product Name | PKC beta 2 antibody [Y125], C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit monoclonal antibody [Y125] to PKC beta IIPKCβII is a member of the serine/threonine protein kinase C (PKC) family that includes 10 closely related isozymes classified as the conventional, the novel and the atypical. The conventional PKCβII is implicated in diverse cellular process including secretion, proliferation, differentiation, apoptosis, permeability, migration & hypertrophy . PKCβII structure consists of a C-terminal catalytic region, containing an activator-binding domain and a Ca2+-binding domain, and a regulatory domain. The catalytic domain is activated by undergoing multiple serine/threonine and tyrosine phosphorylation that are either autocatalytic, or catalyzed by PKC kinases . PKCβII and PKCβI differ only in their C-terminal . |
| Immunogen | A synthetic peptide corresponding to residues near C-terminus of human PKC beta-II was used as immunogen. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | Y125 |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHCIHC-PIPWB |
| For WB: Use at a dilution of 1:500 | |
| Species Reactivity | HumanMonkeyMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PKCbeta, MGC41878, PRKCB, PRKCB2, PKCB, PRKCB1, PKC-beta, PKC beta |
|---|---|
| Target | PKC beta II |
| Molecular Weight(MW) | 77 (note) |
| Tissue Specificity | The antibody does not cross-react with human PKC beta-I. |
Application
C. Immunofluorescent staining of HeLa cells using anti-PKC beta II RabMAb (catalog #).
Application
B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti- PKC beta II RabMAb (catalog #).
Application
A. Western blot analysis on K562 cell lysate using anti-PKC beta II RabMAb (catalog #) dilution 1:500| Application Notes | For WB: Use at a dilution of 1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
