Application
Western blot analysis of PKC delta (pY52) expression in MCF7 serum starvation-treated (A); HeLa PMA-treated (B); Jurkat (C); Raw264.7 serum starvation-treated (D); H9C2 serum starvation-treated (E) whole cell lysates.| Product Name | PKC delta (phospho Tyr52) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PKC delta (phospho Tyr52)Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. The protein encoded by this gene is one of the PKC family members. Studies both in human and mice demonstrate that this kinase is involved in B cell signaling and in the regulation of growth, apoptosis, and differentiation of a variety of cell types. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008] |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human PKC delta. The exact sequence is proprietary. |
| Modification | p-Tyr52 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: 1:500 - 1:1000 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | nPKC-delta , PKCD , protein kinase C, delta , PRKCD , MAY1 |
|---|---|
| Target | PKC delta |
| Tissue Specificity | Recognizes endogenous levels of PKC delta (pY52) protein. |
Application
Western blot analysis of PKC delta (pY52) expression in MCF7 serum starvation-treated (A); HeLa PMA-treated (B); Jurkat (C); Raw264.7 serum starvation-treated (D); H9C2 serum starvation-treated (E) whole cell lysates.| Application Notes | WB: 1:500 - 1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4 C. Upon delivery aliquot and store at -20 C for one year. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
