Application
Lysates prepared from K562 cells stimulated with PMA were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were either left untreated (1-4) or treated with lambda| Product Name | PKC epsilon (phospho Ser729) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to PKC epsilon ( phospho S729 )Protein Kinase C epsilon (PKC epsilon) is an ~87 kDa (migrates predominantly as 95 kDa in quiescient cells) member of the novel group (nPKCs: sensitive to diacylglycerol, phosphatidylserine, and phorbol esters) of the PKC family of serine/threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival, metastasis and transcriptional regulation. PKC epsilon is an actin cytoskeleton regulating protein whose overexpression confers an oncogenic phenotype by affecting the ras signaling cascade. PKC epsilon is phosphorylated by PDK1 on threonine 566 and is then autophosphorylated on the turn motif threonine 710 and the hydrophobic motif serine 729. Catalytically inactive PKC epsilon is not phosphorylated on serine 729. Phosphorylation of serine 729 is mediated by mTOR-dependent pathways, plays an important role in regulating PKC epsilon subcellular localization, and is responsible for the appearance of the 92 kDa PKC epsilon species. |
| Immunogen | Synthetic phosphopeptide derived from a region of human PKC epsilon that contains serine 729. |
| Modification | p-Ser729 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: Use at a dilution of 1/1000. Detects a band of approximately 87 kDa. Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MGC125656, MGC125657, PKCE, nPKC-epsilon, nPKC epsilon, nPKCepsilon, PKC epsilon (phospho S729), PKC epsilon (phospho Ser729), PKC epsilon (pS729), PKC epsilon (pSer729), PKC epsilon phospho S729, PKC epsilon phospho Ser729, Phospho PKC epsilon (S729), Ph |
|---|---|
| Target | PKC epsilon |
| Tissue Specificity | This antibody may partially cross react with beta 2 [pS660] as determined by peptide competition experiments. |
| Cellular Localization | Cytoplasmic |
Application
Lysates prepared from K562 cells stimulated with PMA were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were either left untreated (1-4) or treated with lambda| Application Notes | WB: Use at a dilution of 1/1000. Detects a band of approximately 87 kDa. Optimal dilutions/concentrations should be determined by the end user. |
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| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.05% Sodium Azide; Constituents: 50% Glycerol, PBS, 1mg/ml BSA. pH 7.3 |
