Application
WB analysis of extract from A431 cells, untreated or treated with EGF (200ng/ml, 10 min), using PKD antibody (Lane 1 and 2) and PKD (phospho Ser738) antibody (Lane 3 and 4).Application
ICC/IF analysis of methanol-fixed HeLa cells using PKD (phospho Ser738) antibody (red).| Product Name | PKC mu (phospho Ser738) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to PKC mu (phospho Ser738)PRKD1 is a serine/threonine kinase that regulates a variety of cellular functions, including membrane receptor signaling, transport at the Golgi, protection from oxidative stress at the mitochondria, gene transcription, and regulation of cell shape, motility, and adhesion (summary by Eiseler et al., 2009 [PubMed 19329994]).[supplied by OMIM, Nov 2010] |
| Immunogen | The antiserum was produced against synthesized phosphopeptide derived from human PKD/PKC mu around the phosphorylation site of serine 738 (E -K-SP-F-R). |
| Modification | p-Ser738 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB: 1:500-1:1000 IF:1:100-1:200 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | PKCM , PRKD1 , PKC-MU , protein kinase D1 , PKD , PRKCM |
|---|---|
| Target | PKD (phospho Ser738) |
| Tissue Specificity | PKD/PKC mu (phospho-Ser738) antibody detects endogenous levels of PKD/PKC mu only when phosphorylated at serine 738. |
Application
WB analysis of extract from A431 cells, untreated or treated with EGF (200ng/ml, 10 min), using PKD antibody (Lane 1 and 2) and PKD (phospho Ser738) antibody (Lane 3 and 4).
Application
ICC/IF analysis of methanol-fixed HeLa cells using PKD (phospho Ser738) antibody (red).| Application Notes | WB: 1:500-1:1000 IF:1:100-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C or -80°C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
