Application
Western blot analysis of PKC mu (pY463) expression in HeLa (A) whole cell lysates.Application
Immunohistochemical analysis of PKC mu (pY463) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.| Product Name | PKC mu (phospho Tyr463) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PKC mu (phospho Tyr463)PRKD1 is a serine/threonine kinase that regulates a variety of cellular functions, including membrane receptor signaling, transport at the Golgi, protection from oxidative stress at the mitochondria, gene transcription, and regulation of cell shape, motility, and adhesion (summary by Eiseler et al., 2009 [PubMed 19329994]).[supplied by OMIM, Nov 2010] |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PKC mu (pY463). The exact sequence is proprietary. |
| Modification | p-Tyr463 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| WB: 1:500 - 1:1000. IHC-P: 1:100 - 1:200 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PRKD1 , PKCM , protein kinase D1 , PRKCM , PKC-MU , PKD |
|---|---|
| Target | PKC mu |
| Tissue Specificity | Recognizes endogenous levels of PKC mu (pY463) protein. |
Application
Western blot analysis of PKC mu (pY463) expression in HeLa (A) whole cell lysates.
Application
Immunohistochemical analysis of PKC mu (pY463) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.| Application Notes | WB: 1:500 - 1:1000. IHC-P: 1:100 - 1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4 C. Upon delivery aliquot and store at -20 C for one year. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
