Application
Indirect immunofluorescence analysis of GFP-PKD3 fusion protein expression in Panc-1 cells by using PKD3 C-term antibody.Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary PKD3 C-term antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. HC = heptaocarcinomaApplication
Western blot analysis of anti-PKCnu C-term Pab in NCI-H460 cell lysate. PKCnu (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.Application
'Upper panel, western blot analysis of GFP fusion protein expression in Panc-1 cells by using an anti-GFP antibody. Lane 5: non-transfected cells Lane 6: GFP-PKD-transfected cells Lane 7: GFP-PKD2-transfected cells Lane 8: GFP-PKD3 transfected cells Center panel, western blot analysis of GFP fusion protein expression in Panc-1 cells by using PKD3 C-term antibody. '| Product Name | PKC nu antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to PKC nuProtein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The AGC kinase group consists of 63 kinases including the cyclic nucleotide-regulated protein kinase (PKA & PKG) family, the diacylglycerol-activated/phospholipid-dependent protein kinase C (PKC) family, the related to PKA and PKC (RAC/Akt) protein kinase family, the kinases that phosphorylate G protein-coupled receptors family (ARK), and the kinases that phosphorylate ribosomal protein S6 family (RSK). The calcium/calmodulin-dependent kinase (CAMK) group consists of 75 kinases regulated by Ca2+/CaM and close relative family (CAMK, CAMKL, DAPK, MAPKAPK). |
| Immunogen | Synthetic peptide conjugated to KLH, corresponding to amino acids 875-890 of Human PKC nu. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| ELISA: Use at a dilution of 1/1000. WB: Use at a dilution of 1/100 - 1/500. Detects a band of approximately 100 kDa. Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Protein G |
| Gene Synonyms | EPK2, PKC-NU, PKD3, PRKCN, nPKC-NU, PKC NU, nPKC NU, PKCNU, nPKCNU |
|---|---|
| Target | PKC nu |
Application
Indirect immunofluorescence analysis of GFP-PKD3 fusion protein expression in Panc-1 cells by using PKD3 C-term antibody.
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary PKD3 C-term antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. HC = heptaocarcinoma
Application
Western blot analysis of anti-PKCnu C-term Pab in NCI-H460 cell lysate. PKCnu (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Application
'Upper panel, western blot analysis of GFP fusion protein expression in Panc-1 cells by using an anti-GFP antibody. Lane 5: non-transfected cells Lane 6: GFP-PKD-transfected cells Lane 7: GFP-PKD2-transfected cells Lane 8: GFP-PKD3 transfected cells Center panel, western blot analysis of GFP fusion protein expression in Panc-1 cells by using PKD3 C-term antibody. '| Application Notes | ELISA: Use at a dilution of 1/1000. WB: Use at a dilution of 1/100 - 1/500. Detects a band of approximately 100 kDa. Optimal dilutions/concentrations should be determined by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.09% Sodium Azide; Constituents: PBS |
