| Product Name | PKC theta (phospho Ser695) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to PKC theta ( phospho S695 )Protein Kinase C theta (PKC theta) is an 80 kDa member of the novel group (nPKCs: sensitive to diacylglycerol, phosphatidylserine and phorbol esters) of the PKC family of serine/threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival and homeostasis. Transgenic mice over-expressing dominant negative PKC theta develop hyperinsulinimia. PKC theta is involved in JNK activation and also plays a specialized role in TCR mediated activation of T and B cells. The activation of PKC theta in T cells is associated with its recruitment to the membrane, and is mediated by PI3-kinase and Vav. PKC theta is a constitutively competent kinase and is auto-phosphorylated on serine 676 in the turn loop and serine 695 in the hydrophobic loop. The phosphorylation on serine 695 contributes to the catalytic activity of PKC theta. |
| Immunogen | Synthetic phosphopeptide derived from a region of human PKC theta that contains serine 695. |
| Modification | p-Ser695 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: Use at a concentration of 0.1 - 1.0 μg/ml. Detects a band of approximately 80 kDa. Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | HumanMouse |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MGC126514, MGC141919, PRKCT, nPKC-theta, nPKC theta, nPKCtheta, PKC theta (phospho S695), PKC theta (phospho Ser695), PKC theta (pS695), PKC theta (pSer695), PKC theta phospho S695, PKC theta phospho Ser695, Phospho PKC theta (S695), Phospho PKC theta (Ser |
|---|---|
| Target | PKC theta |
| Tissue Specificity | This antibody does not cross-react with any other PKC isoforms tested. |
| Application Notes | WB: Use at a concentration of 0.1 - 1.0 μg/ml. Detects a band of approximately 80 kDa. Optimal dilutions/concentrations should be determined by the end user. |
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.05% Sodium Azide; Constituents: PBS, 1mg/ml BSA. pH 7.4 |
