Application
IHC-P analysis of human lungcarcinoma tissue using PKC theta (phospho Ser695) antibody (left) or the same antibody pre-incubated with blocking peptide (right).Application
WB analysis of lysates from untreated and PMA-treated 293 cells using PKC theta (phospho Ser695) antibody.| Product Name | PKC theta (phospho Ser695) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to PKC theta (phospho Ser695)Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipid-dependent protein kinase. This kinase is important for T-cell activation. It is required for the activation of the transcription factors NF-kappaB and AP-1, and may link the T cell receptor (TCR) signaling complex to the activation of the transcription factors. [provided by RefSeq, Jul 2008] |
| Immunogen | The antiserum was produced against synthesized phosphopeptide derived from human PKC theta around the phosphorylation site of serine 695. |
| Modification | p-Ser695 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| IHC: 1:50-1:100 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | PRKCQ , PRKCT , protein kinase C, theta , nPKC-theta |
|---|---|
| Target | PKC theta (phospho Ser695) |
| Tissue Specificity | This antibody detects endogenous levels of PKC theta only when phosphorylated at serine 695. |
Application
IHC-P analysis of human lungcarcinoma tissue using PKC theta (phospho Ser695) antibody (left) or the same antibody pre-incubated with blocking peptide (right).
Application
WB analysis of lysates from untreated and PMA-treated 293 cells using PKC theta (phospho Ser695) antibody.| Application Notes | IHC: 1:50-1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C or -80°C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
