Application
Western blot analysis of extracts from PC12 cells using PKC theta Antibody .Application
Immunofluorescence staining of methanol-fixed Hela cells using PKC theta Antibody .Application
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using PKC theta Antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Western blot analysis of extracts from PC12 cells using PKCth(Ab-676) Antibody| Product Name | PKC theta antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PKC thetaThis is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. Required for interleukin-2 (IL2) production. PKC is activated by diacylglycerol. which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. |
| Immunogen | Peptide sequence around aa. 674~678 (R-L-S-F-A) derived from Human PKC theta . |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MGC126514, MGC141919, PRKCT, nPKC-theta, PRKCQ, protein kinase C theta type, protein kinase C, theta |
|---|---|
| Target | PKC theta |
| Molecular Weight(MW) | 80 (note) |
Application
Western blot analysis of extracts from PC12 cells using PKC theta Antibody .
Application
Immunofluorescence staining of methanol-fixed Hela cells using PKC theta Antibody .
Application
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using PKC theta Antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Western blot analysis of extracts from PC12 cells using PKCth(Ab-676) Antibody| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
