Application
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using PKC theta Antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Western blot analysis of extracts from HT29 cells using PKC theta Antibody and the same antibody preincubated with blocking peptide.Application
Western blot analysis of extracts from HT29 cells using PKCth(Ab-695) Antibody and the same antibody preincubated with blocking peptide.| Product Name | PKC theta antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PKC thetaThis is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. Required for interleukin-2 (IL2) production. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. |
| Immunogen | Peptide sequence around aa.693~697 (N-F-S-F-M) derived from Human PKC theta . |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MGC126514, MGC141919, PRKCT, nPKC-theta, PRKCQ, protein kinase C theta type, protein kinase C, theta |
|---|---|
| Target | PKC theta |
| Molecular Weight(MW) | 80 (note) |
Application
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using PKC theta Antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Western blot analysis of extracts from HT29 cells using PKC theta Antibody and the same antibody preincubated with blocking peptide.
Application
Western blot analysis of extracts from HT29 cells using PKCth(Ab-695) Antibody and the same antibody preincubated with blocking peptide.| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
