Application
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC theta antibody diluted at 1:1000.Application
PKC theta antibody detects PKC theta protein at cytoplasm in rat lymph node by immunohistochemical analysis. Sample: Paraffin-embedded rat lymph node. PKC theta antibody diluted at 1:500.Application
PKC theta antibody detects PKC theta protein at cell membrane and cytoplasm in rat spleen by immunohistochemical analysis. Sample: Paraffin-embedded rat spleen. PKC theta antibody diluted at 1:500.| Product Name | PKC theta antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PKC theta (protein kinase C, theta)Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipid-dependent protein kinase. This kinase is important for T-cell activation. It is required for the activation of the transcription factors NF-kappaB and AP-1, and may link the T cell receptor (TCR) signaling complex to the activation of the transcription factors. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human PKC theta. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanRat |
| Predicted Reactivity | Rhesus Monkey, BovineRhesus Monkey (100%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | protein kinase C, theta , PRKCQ , PRKCT , nPKC-theta |
|---|---|
| Target | PKC theta |
| Molecular Weight(MW) | 82 kDa (note) (6) |
Application
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC theta antibody diluted at 1:1000.
Application
PKC theta antibody detects PKC theta protein at cytoplasm in rat lymph node by immunohistochemical analysis. Sample: Paraffin-embedded rat lymph node. PKC theta antibody diluted at 1:500.
Application
PKC theta antibody detects PKC theta protein at cell membrane and cytoplasm in rat spleen by immunohistochemical analysis. Sample: Paraffin-embedded rat spleen. PKC theta antibody diluted at 1:500.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
