Application
Western blot analysis of PKC zeta (pT560) expression in HeLa UV-treated (A); A431 (B); A549 UV-treated (C); SP2/0 UV-treated (D); PC12 UV-treated (E) whole cell lysates.| Product Name | PKC zeta (phospho Thr560) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PKC zeta (phospho Thr560)Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human PKC zeta. The exact sequence is proprietary. |
| Modification | p-Thr560 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: 1:500 - 1:1000 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PRKCZ , PKC-ZETA , protein kinase C, zeta , PKC2 |
|---|---|
| Target | PKC zeta (phospho Thr560) |
| Tissue Specificity | Recognizes endogenous levels of PKC zeta (pT560) protein. |
Application
Western blot analysis of PKC zeta (pT560) expression in HeLa UV-treated (A); A431 (B); A549 UV-treated (C); SP2/0 UV-treated (D); PC12 UV-treated (E) whole cell lysates.| Application Notes | WB: 1:500 - 1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4 °C. Upon delivery aliquot and store at -20 °C for one year. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in 0.42% Potassium phosphate; 0.87% Sodium chloride; pH 7.3; 30% glycerol; and 0.01% sodium azide. |
